Mouse BK (Kininogen-1 Cleaved Into-Kininogen-1 Heavy Chain-Bradykinin-Kininogen-1 Light Chain) Antibody Pair (STJAP00331)
General Information
| Short Description | This BK antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A & Antigen Affinity.Detection Purification: Protein A & Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Kng1 |
| Gene ID | 16644 |
| Uniprot ID | KNG1_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse BK protein.Detection Immunogen: Recombinant Mouse BK protein. |
Additional Info
| Post Translational Modifications | Bradykinin is released from kininogen by plasma kallikrein. Phosphorylated by FAM20C in the extracellular medium. Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus. |
| Function | Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action). |
| Protein Name | Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain |
| Database Links | |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Pair Names | Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain antibody pairKng1 antibody pairKng antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse BK (Kininogen-1 Cleaved Into-Kininogen-1 Heavy Chain-Bradykinin-Kininogen-1 Light Chain) Antibody Pair (STJAP00331)
This antibody pair detects mouse BK (Kininogen-1 cleaved into Kininogen-1 Heavy Chain, Bradykinin, and Kininogen-1 Light Chain), corresponding to UniProt entry O08677. Reactivity is confirmed for mouse samples only.
Both antibodies are rabbit polyclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotin-conjugated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair is validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant mouse BK protein. Both antibodies were purified using Protein A combined with antigen affinity purification.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.