Mouse Beta-EP (Beta-Endorphin) Competitive ELISA Kit (STJE0004324)

Name: Mouse Beta-EP (Beta-Endorphin) Competitive ELISA Kit (STJE0004324)
SKU: STJE0004324
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0004324

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General Information

Short Description	This Beta-EP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	2.5h
Detection	Colormetric

Target Information

Gene Symbol	Pomc
Gene ID	18976
Uniprot ID	COLI_MOUSE
Specificity	This kit recognizes Mouse Beta-EP in samples. No significant cross-reactivity or interference between Mouse Beta-EP and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Specific enzymatic cleavages at paired basic residues yield the different active peptides.
Function	Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate.
Protein Name	Pro-Opiomelanocortin Pomc Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma Gamma-Msh - Corticotropin Adrenocorticotropic Hormone Acth - Melanocyte-Stimulating Hormone Alpha Alpha-Msh Melanotropin Alpha - Corticotropin-Like Intermediary Peptide Clip - Lipotropin Beta Beta-Lph - Lipotropin Gamma Gamma-Lph - Melanocyte-Stimulating Hormone Beta Beta-Msh Melanotropin Beta - Beta-Endorphin - Met-Enkephalin
Database Links	Reactome: R-MMU-111885 Reactome: -MMU-193048 Reactome: -MMU-194002 Reactome: -MMU-202040 Reactome: -MMU-209952 Reactome: -MMU-211976 Reactome: -MMU-375276 Reactome: -MMU-418555 Reactome: -MMU-418594
Cellular Localisation	Secreted Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms
Alternative ELISA Names	Pro-Opiomelanocortin ELISA kit Pomc ELISA kit Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma ELISA kit Gamma-Msh - Corticotropin ELISA kit Adrenocorticotropic Hormone ELISA kit Acth - Melanocyte-Stimulating Hormone Alpha ELISA kit Alpha-Msh ELISA kit Melanotropin Alpha - Corticotropin-Like Intermediary Peptide ELISA kit Clip - Lipotropin Beta ELISA kit Beta-Lph - Lipotropin Gamma ELISA kit Gamma-Lph - Melanocyte-Stimulating Hormone Beta ELISA kit Beta-Msh ELISA kit Melanotropin Beta - Beta-Endorphin - Met-Enkephalin ELISA kit Pomc ELISA kit Pomc1 ELISA kit
Specificity	This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-105	96
EDTA plasma(n=8)	85-101	92
Cell culture media(n=8)	95-107	101

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	49.40	99.80	404.50	51.10	94.90	411.30
Standard deviation	2.90	5.50	14.20	3.20	5.40	14.80
CV (%)	5.87	5.51	3.51	6.26	5.69	3.60

Citations

View product citations for antibody STJE0004324 on CiteAb