Mouse B7-2/CD86 protein (Recombinant) (C-hFc) (STJP002740)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse B7-2/CD86 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Glu245) of mouse CD86/B7-2 (Accession #NP_062261.3) fused with a Fc tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse B7-2/CD86-C-hFc protein was developed in hek293 cells using the region Val24-Glu245. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse CTLA-4/CD152 at 2 Mu g/mL (100 Mu L/well) can bind Mouse B7-2/CD86 with a linear range of 0.1-54.9 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Cd86 |
| Gene ID | 12524 |
| Uniprot ID | CD86_MOUSE |
| Immunogen | Recombinant Mouse B7-2/CD86 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val24-Glu245) of mouse CD86/B7-2 (Accession #NP_062261.3) fused with a Fc tag at the C-terminus. |
| Immunogen Region | Val24-Glu245 |
| Immunogen Sequence | VSVETQAYFNGTAYLPCPFT KAQNISLSELVVFWQDQQKL VLYEHYLGTEKLDSVNAKYL GRTSFDRNNWTLRLHNVQIK DMGSYDCFIQKKPPTGSIIL QQTLTELSVIANFSEPEIKL AQNVTGNSGINLTCTSKQGH PKPKKMYFLITNSTNEYGDN MQISQDNVTELFSISNSLSL SFPDGVWHMTVVCVLETESM KISSKPLNFTQEFPSPQTYW KE |
Additional Info
| Post Translational Modifications | Polyubiquitinated.which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. |
| Protein Name | T-Lymphocyte Activation Antigen Cd86Activation B7-2 AntigenEarly T-Cell Costimulatory Molecule 1Etc-1Cd Antigen Cd86 |
| Database Links | Reactome: R-MMU-1257604Reactome: -MMU-389356Reactome: -MMU-389357Reactome: -MMU-389359Reactome: -MMU-389513Reactome: -MMU-6811558 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | T-Lymphocyte Activation Antigen Cd86 proteinActivation B7-2 Antigen proteinEarly T-Cell Costimulatory Molecule 1 proteinEtc-1 proteinCd Antigen Cd86 proteinCd86 protein |
Information sourced from Uniprot.org