Mouse ANXA5 (Annexin A5) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0001075
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General Information

Short DescriptionThis mouse ANXA5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityMouse
Sensitivity18.75pg/mL
Detection Limit31.25~2000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolAnxa5
Gene ID11747
Uniprot IDANXA5_MOUSE
SpecificityThis kit recognizes Mouse ANXA5 in samples. No significant cross-reactivity or interference between Mouse ANXA5 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Post Translational Modifications S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Protein Name Annexin A5
Anchorin Cii
Annexin V
Annexin-5
Calphobindin I
Cpb-I
Endonexin Ii
Lipocortin V
Placental Anticoagulant Protein 4
Pp4
Placental Anticoagulant Protein I
Pap-I
Thromboplastin Inhibitor
Vascular Anticoagulant-Alpha
Vac-Alpha
Database Links Reactome: R-MMU-114608
Cellular Localisation
Alternative CLIA Names Annexin A5 CLIA kit
Anchorin Cii CLIA kit
Annexin V CLIA kit
Annexin-5 CLIA kit
Calphobindin I CLIA kit
Cpb-I CLIA kit
Endonexin Ii CLIA kit
Lipocortin V CLIA kit
Placental Anticoagulant Protein 4 CLIA kit
Pp4 CLIA kit
Placental Anticoagulant Protein I CLIA kit
Pap-I CLIA kit
Thromboplastin Inhibitor CLIA kit
Vascular Anticoagulant-Alpha CLIA kit
Vac-Alpha CLIA kit
Anxa5 CLIA kit
Anx5 CLIA kit
Specificity This kit recognizes Mouse ANXA5 in samples. No significant cross-reactivity or interference between Mouse ANXA5 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-113 107
EDTA plasma(n=8) 94-107 100
Cell culture media(n=8) 96-110 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 91.90 310.66 684.49 84.23 313.63 741.16
Standard deviation 8.93 29.82 75.98 10.05 29.14 46.84
CV (%) 9.72 9.60 11.10 11.93 9.29 6.32

Citations

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