Mouse ANGPTL3 (Angiopoietin-Related Protein 3) Antibody Pair (STJAP00268)
General Information
| Short Description | This ANGPTL3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Angptl3 |
| Gene ID | 30924 |
| Uniprot ID | ANGL3_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse ANGPTL3 protein.Detection Immunogen: Recombinant Mouse ANGPTL3 protein. |
Additional Info
| Post Translational Modifications | In part proteolytically cleaved by proprotein convertases.proposed to be involved in activation. In primary hepatocytes is intracellularily predominantly processed by FURIN and extracellularily by FURIN and PCSK6/PACE4. In 18.5 dpc embryos 75% of protein is found to be processed compared to 25 % in adults. |
| Function | Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity.the function seems to be specific for the feeding conditions. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface.the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids.the cleaved N-terminal domain is more efficient than the uncleaved proprotein. Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol. Suppresses LPL specifically in oxidative tissues which is required to route very low density lipoprotein (VLDL)-TG to white adipose tissue (WAT) for storage in response to food.the function may involve cooperation with circulating, liver-derived ANGPTL8 and ANGPTL4 expression in WAT. Contributes to lower plasma levels of low density lipoprotein (LDL)-cholesterol by a mechanism that is independent of the canonical pathway implicating APOE and LDLR. May stimulate hypothalamic LPL activity. Involved in angiogenesis. Binds to endothelial cells via integrin alpha-V/beta-3 (ITGAV:ITGB3), activates FAK, MAPK and Akt signaling pathways and induces cell adhesion and cell migration. May increase the motility of podocytes. Secreted from podocytes, may modulate properties of glomerular endothelial cells involving integrin alpha-V/beta-3 and Akt signaling. May induce actin filament rearrangements in podocytes implicating integrin alpha-V/beta-3 and Rac1 activation. Binds to hematopoietic stem cells (HSC) and is involved in the regulation of HSC activity probably implicating down-regulation of IKZF1/IKAROS. |
| Protein Name | Angiopoietin-Related Protein 3Angiopoietin-Like Protein 3 Cleaved Into - Angptl3(17-224 |
| Database Links | Reactome: R-MMU-8963889 |
| Cellular Localisation | SecretedCell ProjectionLamellipodiumColocalized With Hspg2 And Activated Itgb3 On Podocytes |
| Alternative Antibody Pair Names | Angiopoietin-Related Protein 3 antibody pairAngiopoietin-Like Protein 3 Cleaved Into - Angptl3(17-224 antibody pairAngptl3 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse ANGPTL3 (Angiopoietin-Related Protein 3) Antibody Pair (STJAP00268)
This antibody pair detects mouse ANGPTL3 (Angiopoietin-Related Protein 3), corresponding to UniProt entry Q9R182. Reactivity is confirmed for mouse only.
The capture antibody is a rat monoclonal antibody supplied unconjugated at 0.5 mg/mL, while the detection antibody is a goat polyclonal antibody supplied biotinylated at 0.5 mg/mL. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed for this product.
The capture antibody was purified by Protein A or G, and the detection antibody was purified by antigen affinity chromatography. Both antibodies were raised against recombinant mouse ANGPTL3 protein as immunogen.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.