| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR RESEARCH USE ONLY (RUO). |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This Acta2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8 °C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Acta2 |
| Gene ID: |
11475 |
| Uniprot ID: |
ACTA_MOUSE |
| Specificity: |
This kit recognizes Mouse ACT Alpha 2 in samples. No significant cross-reactivity or interference between Mouse ACT Alpha 2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-75 by SETD3. |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| Protein Name | Actin - Aortic Smooth MuscleAlpha-Actin-2 Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form |
| Database Links | Reactome: R-MMU-445355 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative ELISA Names | Actin - Aortic Smooth Muscle ELISA kitAlpha-Actin-2 Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form ELISA kitActa2 ELISA kitActsa ELISA kitActvs ELISA kit |
| Specificity | This kit recognizes Mouse ACTα2 in samples. No significant cross-reactivity or interference between Mouse ACTα2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-107 | 98 |
| EDTA plasma (n=8) | 94-107 | 100 |
| Cell culture media (n=8) | 96-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.09 | 23.93 | 90.86 | 9.81 | 22.84 | 95.39 |
| Standard deviation | 0.55 | 1.17 | 2.91 | 0.55 | 1.26 | 3.09 |
| CV (%) | 5.45 | 4.89 | 3.20 | 5.61 | 5.52 | 3.24 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
