This Acta2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Mouse
Sensitivity
1.88ng/mL
Detection Limit
3.13~200ng/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Mouse ACT Alpha 2 in samples. No significant cross-reactivity or interference between Mouse ACT Alpha 2 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-75 by SETD3. Actin, aortic smooth muscle, intermediate form: N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP.
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name
Actin - Aortic Smooth MuscleAlpha-Actin-2 Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
