Mouse ACAT2 (Acetyl Coenzyme A Acetyltransferase 2) Sandwich ELISA Kit (STJE0006838)
SPECIFICATIONS
General Information
| Short Description | This ACAT2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.047ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | Soat2 |
| Gene ID | 223920 |
| Uniprot ID | SOAT2_MOUSE |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | |
| Post Translational Modifications | Polyubiquitinated by AMFR/gp78 at Cys-280, leading to its degradation when the lipid levels are low. Association with AMFR/gp78 is mediated via interaction with INSIG1. High concentration of cholesterol and fatty acid results in Cys-280 oxidation, preventing ubiquitination at the same site, resulting in protein stabilization. Oxidized at Cys-280: high concentration of cholesterol and fatty acid induce reactive oxygen species, which oxidizes Cys-280, preventing ubiquitination at the same site, and resulting in protein stabilization. |
| Function | Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa. |
| Protein Name | Sterol O-Acyltransferase 2Acyl-Coenzyme A -Cholesterol Acyltransferase 2Acat-2Cholesterol Acyltransferase 2 |
| Database Links | Reactome: R-MMU-8964038 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Sterol O-Acyltransferase 2 ELISA kitAcyl-Coenzyme A -Cholesterol Acyltransferase 2 ELISA kitAcat-2 ELISA kitCholesterol Acyltransferase 2 ELISA kitSoat2 ELISA kitAcact-2 ELISA kitAcat2 ELISA kit |
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Information sourced from Uniprot.org