MAPK3 Positive Control for STJ501703 peptide (STJ505443)

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STJ505443-5

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Applications: WB
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: MAPK3 Positive Control for STJ501703 is synthetically produced from the sequence and is suitable for use in western blot applications.
Formulation: Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range: WB: 1:500
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: MAPK3
Gene ID: 5595
Uniprot ID: MK03_HUMAN
Specificity: This is positive control is recommended for use in combination with MAPK3 antibody STJ501703.
Post Translational Modifications Phosphorylated upon KIT and FLT3 signaling. Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-204. Ubiquitinated by TRIM15 via 'Lys-63'-linked ubiquitination.leading to activation. Deubiquitinated by CYLD.
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC).as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Peptide Name Mitogen-Activated Protein Kinase 3
Map Kinase 3
Mapk 3
Ert2
Extracellular Signal-Regulated Kinase 1
Erk-1
Insulin-Stimulated Map2 Kinase
Map Kinase Isoform P44
P44-Mapk
Microtubule-Associated Protein 2 Kinase
P44-Erk1
Database Links Reactome: R-HSA-110056
Reactome: R-HSA-112409
Reactome: R-HSA-1169408
Reactome: R-HSA-1181150
Reactome: R-HSA-1295596
Reactome: R-HSA-1502540
Reactome: R-HSA-162658 P27361-3
Reactome: R-HSA-170968
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2029482
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559585
Reactome: R-HSA-2871796
Reactome: R-HSA-3371453
Reactome: R-HSA-375165
Reactome: R-HSA-444257
Reactome: R-HSA-445144
Reactome: R-HSA-450341
Reactome: R-HSA-456926
Reactome: R-HSA-5654726
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5663213
Reactome: R-HSA-5668599
Reactome: R-HSA-5673001
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-6811558
Reactome: R-HSA-73728
Reactome: R-HSA-74749
Reactome: R-HSA-877300
Reactome: R-HSA-879415
Reactome: R-HSA-881907
Reactome: R-HSA-8939211
Reactome: R-HSA-8940973
Reactome: R-HSA-8943724
Reactome: R-HSA-9627069
Reactome: R-HSA-9634638
Reactome: R-HSA-9635465
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9656223
Reactome: R-HSA-9664422
Reactome: R-HSA-9725371
Reactome: R-HSA-9732724
Reactome: R-HSA-9768919
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Nucleus
Membrane
Caveola
Cell Junction
Focal Adhesion
Autophosphorylation At Thr-207 Promotes Nuclear Localization
Pea15-Binding Redirects The Biological Outcome Of Mapk3 Kinase-Signaling By Sequestering Mapk3 Into The Cytoplasm
Alternative Peptide Names Mitogen-Activated Protein Kinase 3 protein
Map Kinase 3 protein
Mapk 3 protein
Ert2 protein
Extracellular Signal-Regulated Kinase 1 protein
Erk-1 protein
Insulin-Stimulated Map2 Kinase protein
Map Kinase Isoform P44 protein
P44-Mapk protein
Microtubule-Associated Protein 2 Kinase protein
P44-Erk1 protein
MAPK3 protein
ERK1 protein
PRKM3 protein

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance