| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
MAPK10 Positive Control for STJ501683 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
MAPK10 |
| Gene ID: |
5602 |
| Uniprot ID: |
MK10_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with MAPK10 antibody STJ501683. |
| Tissue Specificity | Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney. |
| Post Translational Modifications | Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. Palmitoylation regulates subcellular location and axonal development. |
| Function | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. |
| Peptide Name | Mitogen-Activated Protein Kinase 10Map Kinase 10Mapk 10Map Kinase P49 3f12Stress-Activated Protein Kinase 1bSapk1bStress-Activated Protein Kinase Jnk3C-Jun N-Terminal Kinase 3 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-450341 |
| Cellular Localisation | CytoplasmMembraneLipid-AnchorNucleusMitochondrionPalmitoylation Regulates Mapk10 Trafficking To CytoskeletonRecruited To The Mitochondria In The Presence Of Sarm1 |
| Alternative Peptide Names | Mitogen-Activated Protein Kinase 10 proteinMap Kinase 10 proteinMapk 10 proteinMap Kinase P49 3f12 proteinStress-Activated Protein Kinase 1b proteinSapk1b proteinStress-Activated Protein Kinase Jnk3 proteinC-Jun N-Terminal Kinase 3 proteinMAPK10 proteinJNK3 proteinJNK3A proteinPRKM10 proteinSAPK1B protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
