| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
MAP3K14 Positive Control for STJ501669 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
MAP3K14 |
| Gene ID: |
9020 |
| Uniprot ID: |
M3K14_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with MAP3K14 antibody STJ501669. |
| Tissue Specificity | Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. |
| Post Translational Modifications | Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. |
| Function | Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. |
| Peptide Name | Mitogen-Activated Protein Kinase Kinase Kinase 14Nf-Kappa-Beta-Inducing KinaseHsnikSerine/Threonine-Protein Kinase Nik |
| Database Links | Reactome: R-HSA-389357Reactome: R-HSA-5607761Reactome: R-HSA-5668541Reactome: R-HSA-5676590Reactome: R-HSA-5676594 |
| Cellular Localisation | Cytoplasm |
| Alternative Peptide Names | Mitogen-Activated Protein Kinase Kinase Kinase 14 proteinNf-Kappa-Beta-Inducing Kinase proteinHsnik proteinSerine/Threonine-Protein Kinase Nik proteinMAP3K14 proteinNIK protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
