Ready-To-Use

ELISA Kits

Ready-To-Use ELISA Kits

Over 4,000 pre-configured sandwich and competitive kits, supplied at 48 T or 96 T.

Our Ready-To-Use range covers both sandwich and competitive ELISA formats across a wide set of species, so a study can move from target selection straight to plate setup without a separate kit-development step. Every kit ships with its own downloadable datasheet and safety data sheet.

How it works

Standard kit vs. Ready-To-Use kit

Ready-To-Use applies to both our sandwich and competitive kits. In each format, the detection reagents are supplied pre-diluted to working strength instead of as a concentrate you dilute yourself. The effect on total assay time differs by format, as shown below.

Sandwich ELISA kits

Kit component Standard kit Ready-To-Use kit
Detection antibody Supplied as a 100x concentrate. Diluted 1:99 with buffer before use. Supplied pre-diluted as Detection Solution A. Added directly, no dilution step.
HRP enzyme conjugate Supplied as a 100x concentrate. Diluted 1:99 with buffer before use. Supplied pre-diluted as Detection Solution B. Added directly, no dilution step.
Assay time 4.5 hours 3 hours
Wash buffer 30x concentrate, diluted 1:29 before use 30x concentrate, diluted 1:29 before use
Standard Lyophilized, reconstituted before use Lyophilized, reconstituted before use

Competitive ELISA kits

Kit component Standard kit Ready-To-Use kit
Detection Reagent A Diluted 1:99 with buffer to prepare a working solution. Supplied at the correct working concentration. No dilution required.
Detection Reagent B Diluted 1:99 with buffer to prepare a working solution. Supplied at the correct working concentration. No dilution required.
Assay time 2.5 hours 2.5 hours (unchanged; the dilution step is removed, not the incubation time)
Wash buffer 30x concentrate, diluted 1:29 before use 30x concentrate, diluted 1:29 before use
Standard Lyophilized, reconstituted before use Lyophilized, reconstituted before use
Other ELISA formats

Choosing between sandwich and competitive formats

Sandwich ELISA Kits

For larger antigens, our matched capture and detection antibody pairs quantify targets with high sensitivity and specificity.

View sandwich ELISA kits 		Competitive ELISA Kits

For small molecules, hormones and metabolites, our single-antibody competitive kits use signal interference detection.

View competitive ELISA kits

Need help choosing a Ready-To-Use kit?

Please allow up to 10 working days for kit dispatch. Kits are sent on overnight priority shipping with gel ice packs. See individual datasheets for confirmed sensitivity, range and cross-reactivity data.

Looking for more ELISA kits?

Browse the full catalogue of over 4,000 kits, spanning sandwich, competitive and antibody pair formats.

 Browse all ELISA kits

For Research Use Only. Not for use in diagnostic procedures.

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