Ready-To-Use

ELISA Kits

Ready-To-Use ELISA Kits

Over 4,000 pre-configured sandwich and competitive kits, supplied at 48 T or 96 T.

Our Ready-To-Use range covers both sandwich and competitive ELISA formats across a wide set of species, so a study can move from target selection straight to plate setup without a separate kit-development step. Every kit ships with its own downloadable datasheet and safety data sheet.

Explore ELISA Kits research insights Browse Ready-To-Use kits on this page

How it works

Standard kit vs. Ready-To-Use kit

Ready-To-Use applies to both our sandwich and competitive kits. In each format, the detection reagents are supplied pre-diluted to working strength instead of as a concentrate you dilute yourself. The effect on total assay time differs by format, as shown below.

Sandwich ELISA kits

Kit component	Standard kit	Ready-To-Use kit
Detection antibody	Supplied as a 100x concentrate. Diluted 1:99 with buffer before use.	Supplied pre-diluted as Detection Solution A. Added directly, no dilution step.
HRP enzyme conjugate	Supplied as a 100x concentrate. Diluted 1:99 with buffer before use.	Supplied pre-diluted as Detection Solution B. Added directly, no dilution step.
Assay time	4.5 hours	3 hours
Wash buffer	30x concentrate, diluted 1:29 before use	30x concentrate, diluted 1:29 before use
Standard	Lyophilized, reconstituted before use	Lyophilized, reconstituted before use

Competitive ELISA kits

Kit component	Standard kit	Ready-To-Use kit
Detection Reagent A	Diluted 1:99 with buffer to prepare a working solution.	Supplied at the correct working concentration. No dilution required.
Detection Reagent B	Diluted 1:99 with buffer to prepare a working solution.	Supplied at the correct working concentration. No dilution required.
Assay time	2.5 hours	2.5 hours (unchanged; the dilution step is removed, not the incubation time)
Wash buffer	30x concentrate, diluted 1:29 before use	30x concentrate, diluted 1:29 before use
Standard	Lyophilized, reconstituted before use	Lyophilized, reconstituted before use

Other ELISA formats

Choosing between sandwich and competitive formats

Sandwich ELISA Kits

For larger antigens, our matched capture and detection antibody pairs quantify targets with high sensitivity and specificity.

View sandwich ELISA kits

Competitive ELISA Kits

For small molecules, hormones and metabolites, our single-antibody competitive kits use signal interference detection.

View competitive ELISA kits

Need help choosing a Ready-To-Use kit?

Please allow up to 10 working days for kit dispatch. Kits are sent on overnight priority shipping with gel ice packs. See individual datasheets for confirmed sensitivity, range and cross-reactivity data.