iNOS Blocking Peptide is synthetically produced from the 1064-1114 sequence and is suitable for use in western blot applications.
Applications
Immunodepletion/Immunocompetition
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Formulation
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice.
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Synthetic peptide taken within amino acid region 1064-1114 on human inducible nitric oxide synthase protein.
Immunogen Region
1064-1114
Additional Info
Tissue Specificity
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Expressed in chondrocytes.
Post Translational Modifications
Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
Peptide Name
Nitric Oxide Synthase - InducibleHepatocyte NosHep-NosInducible No SynthaseInducible NosInosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2
CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization
Alternative Peptide Names
Nitric Oxide Synthase - Inducible proteinHepatocyte Nos proteinHep-Nos proteinInducible No Synthase proteinInducible Nos proteinInos proteinNos Type Ii proteinPeptidyl-Cysteine S-Nitrosylase Nos2 proteinNOS2 proteinNOS2A protein
