| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
HYPE Blocking Peptide for STJ506116 is synthetically produced from the 400-450 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
FICD |
| Gene ID: |
11153 |
| Uniprot ID: |
FICD_HUMAN |
| Immunogen Region: |
400-450 |
| Specificity: |
This blocking peptide is recommended for use in combination with HYPE antibody, STJ506116 |
| Immunogen: |
Synthetic peptide corresponding to amino acids within region 400-450 on mouse Protein adenylyltransferase FICD. |
| Tissue Specificity | Ubiquitous. |
| Post Translational Modifications | Auto-AMPylated in vitro.it is unclear whether auto-AMPylation is relevant in vivo. N-glycosylated.predominantly glycosylated at Asn-275. |
| Function | Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates. |
| Peptide Name | Protein Adenylyltransferase FicdAmpylator FicdDe-Ampylase FicdFic Domain-Containing ProteinHuntingtin Yeast Partner EHuntingtin-Interacting Protein 13Hip-13Huntingtin-Interacting Protein E |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Peptide Names | Protein Adenylyltransferase Ficd proteinAmpylator Ficd proteinDe-Ampylase Ficd proteinFic Domain-Containing Protein proteinHuntingtin Yeast Partner E proteinHuntingtin-Interacting Protein 13 proteinHip-13 proteinHuntingtin-Interacting Protein E proteinFICD proteinHIP13 proteinHYPE proteinUNQ3041 proteinPRO9857 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
