Human YES1 protein (Recombinant) (N-His) (STJP008785)

SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
STJP008785
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General Information

Short DescriptionRecombinant-Human YES1-N-His protein was developed from e.coli for the region N-His. For use in research applications.
ApplicationsELISA/Immunogen/SDS-PAGE/WB
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Dilution RangeReconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
FormulationLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage InstructionUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.

Target Information

Gene SymbolYES1
Gene ID7525
Uniprot IDYES_HUMAN
ImmunogenHomo sapiens (Human)
Immunogen RegionThr94-Leu543

Additional Info

Post Translational Modifications Phosphorylated. Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-426 maintains enzyme activity by blocking CSK-mediated inhibition. Palmitoylation at Cys-3 promotes membrane localization.
Function Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity.
Protein Name Tyrosine-Protein Kinase Yes
Proto-Oncogene C-Yes
P61-Yes
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1433557
Reactome: R-HSA-1433559
Reactome: R-HSA-2029481
Reactome: R-HSA-210990
Reactome: R-HSA-2682334
Reactome: R-HSA-389356
Reactome: R-HSA-389513
Reactome: R-HSA-3928662
Reactome: R-HSA-3928663
Reactome: R-HSA-3928665
Reactome: R-HSA-8940973
Reactome: R-HSA-912631
Reactome: R-HSA-9664323
Reactome: R-HSA-9664422
Reactome: R-HSA-9670439
Reactome: R-HSA-9680350
Cellular Localisation Cell Membrane
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cytosol
Cell Junction
Newly Synthesized Protein Initially Accumulates In The Golgi Region And Traffics To The Plasma Membrane Through The Exocytic Pathway
Localized To Small Puncta Throughout The Cytoplasm And Cell Membrane When In The Presence Of Snail1
Alternative Protein Names Tyrosine-Protein Kinase Yes protein
Proto-Oncogene C-Yes protein
P61-Yes protein
YES1 protein
YES protein

Information sourced from Uniprot.org

Citations

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