Human VWF (Von Willebrand Factor) Antibody Pair (STJAP00179)
General Information
| Short Description | This VWF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | VWF |
| Gene ID | 7450 |
| Uniprot ID | VWF_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human VWF protein.Detection Immunogen: Recombinant Human VWF protein. |
Additional Info
| Post Translational Modifications | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. |
| Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. |
| Protein Name | Von Willebrand FactorVwf Cleaved Into - Von Willebrand Antigen 2Von Willebrand Antigen Ii |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140837Reactome: R-HSA-216083Reactome: R-HSA-354192Reactome: R-HSA-354194Reactome: R-HSA-372708Reactome: R-HSA-430116Reactome: R-HSA-5674135Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-75892Reactome: R-HSA-76009Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9672391Reactome: R-HSA-9672393Reactome: R-HSA-9845619Reactome: R-HSA-9845620Reactome: R-HSA-9845621Reactome: R-HSA-9845622Reactome: R-HSA-9846298 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixLocalized To Storage Granules |
| Alternative Antibody Pair Names | Von Willebrand Factor antibody pairVwf Cleaved Into - Von Willebrand Antigen 2 antibody pairVon Willebrand Antigen Ii antibody pairVWF antibody pairF8VWF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human VWF (Von Willebrand Factor) Antibody Pair (STJAP00179)
This antibody pair detects human Von Willebrand Factor (VWF), corresponding to UniProt entry P04275. Reactivity is confirmed for human samples only.
The capture antibody is an unconjugated mouse monoclonal antibody and the detection antibody is a biotin-conjugated rabbit monoclonal antibody. Both are supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human VWF protein. Both antibodies were purified by Protein A chromatography.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.