Human VLDL receptor protein (Recombinant) (STJP000581)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding extracellular domain of Human Very Low density lipoprotein receptor including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human VLDL receptor-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by the ability of immobilized recombinant Human Apolipoprotein E3 to bind recombinant human VLDLR in ELISA. The ED50 was calculated to be 30-100ng/ml. NA |
Target Information
| Gene Symbol | VLDLR |
| Gene ID | 7436 |
| Uniprot ID | VLDLR_HUMAN |
| Immunogen | Optimized DNA sequence encoding extracellular domain of Human Very Low density lipoprotein receptor including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ECD |
Additional Info
| Tissue Specificity | Abundant in heart and skeletal muscle.also ovary and kidney.not in liver. |
| Post Translational Modifications | Ubiquitinated at Lys-839 by MYLIP leading to degradation. Glycosylated. |
| Function | Multifunctional cell surface receptor that binds VLDL and transports it into cells by endocytosis and therefore plays an important role in energy metabolism. Also binds to a wide range of other molecules including Reelin/RELN or apolipoprotein E/APOE-containing ligands as well as clusterin/CLU. In the off-state of the pathway, forms homooligomers or heterooligomers with LRP8. Upon binding to ligands, homooligomers are rearranged to higher order receptor clusters that transmit the extracellular RELN signal to intracellular signaling processes by binding to DAB1. This interaction results in phosphorylation of DAB1 leading to the ultimate cell responses required for the correct positioning of newly generated neurons. Later, mediates a stop signal for migrating neurons, preventing them from entering the marginal zone. (Microbial infection) Acts as a receptor for Semliki Forest virus. |
| Protein Name | Very Low-Density Lipoprotein ReceptorVldl ReceptorVldl-R |
| Database Links | Reactome: R-HSA-8866376Reactome: R-HSA-8866427Reactome: R-HSA-8964046 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembraneClathrin-Coated Pit |
| Alternative Protein Names | Very Low-Density Lipoprotein Receptor proteinVldl Receptor proteinVldl-R proteinVLDLR protein |
Information sourced from Uniprot.org