Human VF (Nicotinamide Phosphoribosyltransferase) Antibody Pair (STJAP00063)
General Information
| Short Description | This VF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | NAMPT |
| Gene ID | 10135 |
| Uniprot ID | NAMPT_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human VF protein.Detection Immunogen: Recombinant Human VF protein. |
Additional Info
| Function | Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression. |
| Protein Name | Nicotinamide PhosphoribosyltransferaseNamprtaseNamptPre-B-Cell Colony-Enhancing Factor 1Pre-B Cell-Enhancing FactorVisfatin |
| Database Links | Reactome: R-HSA-1368108Reactome: R-HSA-197264Reactome: R-HSA-9768919 |
| Cellular Localisation | NucleusCytoplasmSecretedUnder Non-Inflammatory ConditionsVisfatin Predominantly Exhibits A Granular Pattern Within The NucleusSecreted By Endothelial Cells Upon Il-1beta StimulationAbundantly Secreted In MilkReaching 100-Fold Higher Concentrations Compared To Maternal Serum |
| Alternative Antibody Pair Names | Nicotinamide Phosphoribosyltransferase antibody pairNamprtase antibody pairNampt antibody pairPre-B-Cell Colony-Enhancing Factor 1 antibody pairPre-B Cell-Enhancing Factor antibody pairVisfatin antibody pairNAMPT antibody pairPBEF antibody pairPBEF1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human VF (Nicotinamide Phosphoribosyltransferase) Antibody Pair (STJAP00063)
This antibody pair detects human Nicotinamide Phosphoribosyltransferase (NAMPT, also known as VISFATIN), corresponding to UniProt entry P43490. Reactivity is confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are mouse monoclonal antibodies supplied at 0.5 mg/mL, purified by Protein A or G affinity chromatography.
This pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant human NAMPT/VISFATIN protein. No further detail on immunogen region is provided.
Both antibodies should be stored at -20 degrees Celsius for long-term storage, and freeze-thaw cycles should be avoided. Both components are formulated in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.4.
No. This product is for research use only and is not intended for diagnostic, therapeutic, or clinical applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.