Human VEGFR2 protein (Recombinant) (STJP000525)

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STJP000525

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human VEGFR2-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by the ability to inhibit proliferation of HUVEC cells. The ED50 measured for this effect i 10-20 ng/ml NA
Gene Symbol: KDR
Gene ID: 3791
Uniprot ID: VGFR2_HUMAN
Immunogen Region: MET1-GLU764
Immunogen: Optimized DNA sequence encoding mature chain of human Vascular endothelial growth factor receptor 2 (VEGFR2) (MET1-GLU764) including an C-terminal His tag was expressed in HEK293 cells. NA
Tissue Specificity Detected in cornea (at protein level). Widely expressed.
Post Translational Modifications N-glycosylated. Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-951 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1175 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1214 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. The inhibitory disulfide bond between Cys-1024 and Cys-1045 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function.
Function Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Protein Name Vascular Endothelial Growth Factor Receptor 2
Vegfr-2
Fetal Liver Kinase 1
Flk-1
Kinase Insert Domain Receptor
Kdr
Protein-Tyrosine Kinase Receptor Flk-1
Cd Antigen Cd309
Database Links Reactome: R-HSA-194306
Reactome: R-HSA-195399
Reactome: R-HSA-216083
Reactome: R-HSA-4420097
Reactome: R-HSA-5218921
Reactome: R-HSA-9673768
Cellular Localisation Cell Junction
Endoplasmic Reticulum
Cell Membrane
Localized With Rap1a At Cell-Cell Junctions
Colocalizes With Ern1 And Xbp1 In The Endoplasmic Reticulum In Endothelial Cells In A Vascular Endothelial Growth Factor (Vegf)-Dependent Manner
Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Nucleus
Cytoplasmic Vesicle
Early Endosome
Detected On Caveolae-Enriched Lipid Rafts At The Cell Surface
Is Recycled From The Plasma Membrane To Endosomes And Back Again
Phosphorylation Triggered By Vegfa Binding Promotes Internalization And Subsequent Degradation
Vegfa Binding Triggers Internalization And Translocation To The Nucleus
Isoform 2: Secreted
Isoform 3: Secreted
Alternative Protein Names Vascular Endothelial Growth Factor Receptor 2 protein
Vegfr-2 protein
Fetal Liver Kinase 1 protein
Flk-1 protein
Kinase Insert Domain Receptor protein
Kdr protein
Protein-Tyrosine Kinase Receptor Flk-1 protein
Cd Antigen Cd309 protein
KDR protein
FLK1 protein
VEGFR2 protein

Information sourced from Uniprot.org

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