Human VEGFB (Vascular endothelial growth factor B) Sandwich ELISA Kit (STJE0003500)

Name: Human VEGFB (Vascular endothelial growth factor B) Sandwich ELISA Kit (STJE0003500)
SKU: STJE0003500
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003500

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Short Description	This VEGFB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	VEGFB
Gene ID	7423
Uniprot ID	VEGFB_HUMAN
Specificity	This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	VEGF-B186 is O-glycosylated.
Function	Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Protein Name	Vascular Endothelial Growth Factor B Vegf-B Vegf-Related Factor Vrf
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-194313 Reactome: R-HSA-195399
Cellular Localisation	Secreted Secreted But Remains Associated To Cells Or To The Extracellular Matrix Unless Released By Heparin
Alternative ELISA Names	Vascular Endothelial Growth Factor B ELISA kit Vegf-B ELISA kit Vegf-Related Factor ELISA kit Vrf ELISA kit VEGFB ELISA kit VRF ELISA kit
Specificity	This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	51.07	102.94	400.36	51.34	106.20	365.76
Standard deviation	2.75	4.67	13.05	3.14	4.59	16.06
CV (%)	5.38	4.54	3.26	6.12	4.32	4.39