This VEGF165 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Human
Sensitivity
18.75pg/mL
Detection Limit
31.25~2000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human VEGF165 in samples. No significant cross-reactivity or interference between Human VEGF165 and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
Function
N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Protein Name
Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa
N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf189: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf121: SecretedIsoform Vegf165: SecretedIsoform Vegf189: SecretedCell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin
Alternative ELISA Names
Vascular Endothelial Growth Factor A - Long Form ELISA kitL-Vegf ELISA kitVascular Permeability Factor ELISA kitVpf Cleaved Into - N-Vegf - Vegfa ELISA kitVEGFA ELISA kitVEGF ELISA kit
Specificity
This kit recognizes Human VEGF165 in samples. No significant cross-reactivity or interference between Human VEGF165 and analogues was observed.
