| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human VEGF121-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Conjugation: |
Biotin |
| Formulation: |
Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: |
Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Storage Instruction: |
The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
| Gene Symbol: |
VEGFA |
| Gene ID: |
7422 |
| Uniprot ID: |
VEGFA_HUMAN |
| Immunogen Region: |
Ala27-Arg147 |
| Immunogen: |
DNA sequence encoding Human VEGF121 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1234158Reactome: R-HSA-194138Reactome: R-HSA-194313Reactome: R-HSA-195399Reactome: R-HSA-4420097 P15692-4Reactome: R-HSA-5218921 P15692-4Reactome: R-HSA-6785807Reactome: R-HSA-8866910Reactome: R-HSA-9679191 P15692-4 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf189: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf121: SecretedIsoform Vegf165: SecretedIsoform Vegf189: SecretedCell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative Protein Names | Vascular Endothelial Growth Factor A - Long Form proteinL-Vegf proteinVascular Permeability Factor proteinVpf Cleaved Into - N-Vegf - Vegfa proteinVEGFA proteinVEGF protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
