| Host: |
E. coli |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human VEGF-A/VEGF121 (K321N)-No tag protein was developed from e. coli and has a target region of No tag. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of 20mM TRIS, 100mM NaCl, pH 8.0. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Human VEGF121 at 2 Mu g/mL (100 Mu L/well) can bind Human KDR with a linear range of 0.2-10 ng/mL.2. Measured in a cell proliferation assay using human umbilical vein endothelial cell |
| Gene Symbol: |
VEGFA |
| Gene ID: |
7422 |
| Uniprot ID: |
VEGFA_HUMAN |
| Immunogen: |
Recombinant Human VEGF-A/VEGF121 (K321N) Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala207-Arg327 (Lys321Asn) ) of human VEGF121 (Accession #NP_001020541.2). |
| Tissue Specificity | Higher expression in pituitary tumors than the pituitary gland. Isoform VEGF189: Widely expressed. Isoform VEGF165: Widely expressed. Isoform VEGF121: Widely expressed. Isoform VEGF206: Not widely expressed. Isoform VEGF145: Not widely expressed. |
| Post Translational Modifications | Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| Function | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Protein Name | Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1234158Reactome: R-HSA-194138Reactome: R-HSA-194313Reactome: R-HSA-195399Reactome: R-HSA-4420097 P15692-4Reactome: R-HSA-5218921 P15692-4Reactome: R-HSA-6785807Reactome: R-HSA-8866910Reactome: R-HSA-9679191 P15692-4 |
| Cellular Localisation | N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf189: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf121: SecretedIsoform Vegf165: SecretedIsoform Vegf189: SecretedCell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin |
| Alternative Protein Names | Vascular Endothelial Growth Factor A - Long Form proteinL-Vegf proteinVascular Permeability Factor proteinVpf Cleaved Into - N-Vegf - Vegfa proteinVEGFA proteinVEGF protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
