Human VCAN (Versican core protein) Sandwich ELISA Kit (STJE0002814)

Name: Human VCAN (Versican core protein) Sandwich ELISA Kit (STJE0002814)
SKU: STJE0002814
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002814

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General Information

Short Description	This VCAN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.19ng/mL
Detection Limit	0.31~20ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	VCAN
Gene ID	1462
Uniprot ID	CSPG2_HUMAN
Specificity	This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Phosphorylated by FAM20C in the extracellular medium. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration.
Function	May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Protein Name	Versican Core Protein Chondroitin Sulfate Proteoglycan Core Protein 2 Chondroitin Sulfate Proteoglycan 2 Glial Hyaluronate-Binding Protein Ghap Large Fibroblast Proteoglycan Pg-M
Database Links	Reactome: R-HSA-1971475 Reactome: R-HSA-2022870 Reactome: R-HSA-2022923 Reactome: R-HSA-2024101 Reactome: R-HSA-3000178 Reactome: R-HSA-3560783 Reactome: R-HSA-3560801 Reactome: R-HSA-3595172 Reactome: R-HSA-3595174 Reactome: R-HSA-3595177 Reactome: R-HSA-381426 Reactome: R-HSA-4420332 Reactome: R-HSA-8957275
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Cell Projection Cilium Photoreceptor Outer Segment Interphotoreceptor Matrix
Alternative ELISA Names	Versican Core Protein ELISA kit Chondroitin Sulfate Proteoglycan Core Protein 2 ELISA kit Chondroitin Sulfate Proteoglycan 2 ELISA kit Glial Hyaluronate-Binding Protein ELISA kit Ghap ELISA kit Large Fibroblast Proteoglycan ELISA kit Pg-M ELISA kit VCAN ELISA kit CSPG2 ELISA kit
Specificity	This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-98	92
EDTA plasma (n=8)	92-104	98
Cell culture media (n=8)	88-101	94

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.92	2.97	8.70	0.87	3.16	9.15
Standard deviation	0.05	0.13	0.39	0.06	0.17	0.38
CV (%)	5.43	4.38	4.48	6.90	5.38	4.15

Citations

View product citations for antibody STJE0002814 on CiteAb