| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This VCAN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
VCAN |
| Gene ID: |
1462 |
| Uniprot ID: |
CSPG2_HUMAN |
| Specificity: |
This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. |
| Function | May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. |
| Protein Name | Versican Core ProteinChondroitin Sulfate Proteoglycan Core Protein 2Chondroitin Sulfate Proteoglycan 2Glial Hyaluronate-Binding ProteinGhapLarge Fibroblast ProteoglycanPg-M |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022870Reactome: R-HSA-2022923Reactome: R-HSA-2024101Reactome: R-HSA-3000178Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3595172Reactome: R-HSA-3595174Reactome: R-HSA-3595177Reactome: R-HSA-381426Reactome: R-HSA-4420332Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixCell ProjectionCiliumPhotoreceptor Outer SegmentInterphotoreceptor Matrix |
| Alternative ELISA Names | Versican Core Protein ELISA kitChondroitin Sulfate Proteoglycan Core Protein 2 ELISA kitChondroitin Sulfate Proteoglycan 2 ELISA kitGlial Hyaluronate-Binding Protein ELISA kitGhap ELISA kitLarge Fibroblast Proteoglycan ELISA kitPg-M ELISA kitVCAN ELISA kitCSPG2 ELISA kit |
| Specificity | This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-98 | 92 |
| EDTA plasma (n=8) | 92-104 | 98 |
| Cell culture media (n=8) | 88-101 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.92 | 2.97 | 8.70 | 0.87 | 3.16 | 9.15 |
| Standard deviation | 0.05 | 0.13 | 0.39 | 0.06 | 0.17 | 0.38 |
| CV (%) | 5.43 | 4.38 | 4.48 | 6.90 | 5.38 | 4.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
