Human VCAM1 (VCAM1) Antibody Pair (STJAP00925)
General Information
| Short Description | This VCAM1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-9D-2/R03-9L-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | VCAM1 |
| Gene ID | 7412 |
| Uniprot ID | VCAM1_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. |
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1V-Cam 1Vcam-1Incam-100Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-6785807Reactome: R-HSA-877300 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative Antibody Pair Names | Vascular Cell Adhesion Protein 1 antibody pairV-Cam 1 antibody pairVcam-1 antibody pairIncam-100 antibody pairCd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 antibody pairVCAM1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human VCAM1 (VCAM1) Antibody Pair (STJAP00925)
This antibody pair detects human VCAM1 (Vascular Cell Adhesion Molecule 1, UniProt P19320). Reactivity is confirmed for human samples only.
This antibody pair is validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both antibodies are rabbit monoclonal IgGs (clone IDs R06-9D-2 and R03-9L-1) supplied unconjugated in 0.01M PBS at pH 7.2, and purified by affinity chromatography. Neither antibody carries a conjugate or tag, so end users are responsible for labeling the detection antibody as required by their assay setup.
Both antibodies in this pair were raised against a recombinant human VCAM1 protein. No additional immunogen region information is provided in the product specification.
Working concentrations and dilutions are not predefined for this product and must be determined empirically by the end user based on their specific experimental conditions and assay format.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.