Human VCAM-1/CD106 (Vascular Cell Adhesion Protein 1) Antibody Pair (STJAP00426)
General Information
| Short Description | This VCAM-1/CD106 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20Β°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | VCAM1 |
| Gene ID | 7412 |
| Uniprot ID | VCAM1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human VCAM-1/CD106 protien.Detection Immunogen: Recombinant Human VCAM-1/CD106 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1V-Cam 1Vcam-1Incam-100Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Database Links | Reactome: R-HSA-198933Reactome: R-HSA-216083Reactome: R-HSA-6785807Reactome: R-HSA-877300 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative Antibody Pair Names | Vascular Cell Adhesion Protein 1 antibody pairV-Cam 1 antibody pairVcam-1 antibody pairIncam-100 antibody pairCd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 antibody pairVCAM1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human VCAM-1/CD106 (Vascular Cell Adhesion Protein 1) Antibody Pair (STJAP00426)
This antibody pair detects human VCAM-1/CD106 (Vascular Cell Adhesion Protein 1, UniProt P19320) and has confirmed reactivity with human samples only. It is not validated for use with samples from other species.
This antibody pair is validated for use in ELISA. Compatible sample types include serum, plasma, and other biological fluids.
Both antibodies are supplied at 0.5 mg/mL and are raised in mouse. The capture antibody is unconjugated, while the detection antibody is biotin-conjugated. Both are purified by Protein A or G.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both antibodies were raised against recombinant human VCAM-1/CD106 protein. No further details on the specific immunogen region are provided in the current product specification.
Both components should be stored at -20Β°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.