Human USP33 protein (Recombinant) (N-His-SUMO & C-Strep) (STJP010983)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human USP33-N-His-SUMO & C-Strep protein was developed from e.coli for the region N-His-SUMO & C-Strep. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | USP33 |
| Gene ID | 23032 |
| Uniprot ID | UBP33_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg36-Glu120 |
Additional Info
| Post Translational Modifications | Ubiquitinated via a VHL-dependent pathway for proteasomal degradation. |
| Function | Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
| Protein Name | Ubiquitin Carboxyl-Terminal Hydrolase 33Deubiquitinating Enzyme 33Ubiquitin Thioesterase 33Ubiquitin-Specific-Processing Protease 33Vhl-Interacting Deubiquitinating Enzyme 1Hvdu1 |
| Database Links | Reactome: R-HSA-5689880Reactome: R-HSA-9010553 |
| Cellular Localisation | CytoplasmPerinuclear RegionCytoskeletonMicrotubule Organizing CenterCentrosomeAssociates With Centrosomes Predominantly In S And G2 Phases But Less In G1 PhaseIsoform 3: Golgi Apparatus |
| Alternative Protein Names | Ubiquitin Carboxyl-Terminal Hydrolase 33 proteinDeubiquitinating Enzyme 33 proteinUbiquitin Thioesterase 33 proteinUbiquitin-Specific-Processing Protease 33 proteinVhl-Interacting Deubiquitinating Enzyme 1 proteinHvdu1 proteinUSP33 proteinKIAA1097 proteinVDU1 protein |
Information sourced from Uniprot.org