Human Urokinase-type plasminogen activator protein (Recombinant) (STJP001870)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding peptidase domain of Human uPA (SER21-LEU431) including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Urokinase-type plasminogen activator-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by the ability of cleave the fluorogenic peptide substrate and was measured to be >2 nMoles/min/ug NA |
Target Information
| Gene Symbol | PLAU |
| Gene ID | 5328 |
| Uniprot ID | UROK_HUMAN |
| Immunogen | Optimized DNA sequence encoding peptidase domain of Human uPA (SER21-LEU431) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | SER21-LEU431 |
Additional Info
| Tissue Specificity | Expressed in the prostate gland and prostate cancers. |
| Post Translational Modifications | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4. |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. |
| Protein Name | Urokinase-Type Plasminogen ActivatorU-Plasminogen ActivatorUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-75205 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Urokinase-Type Plasminogen Activator proteinU-Plasminogen Activator proteinUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B proteinPLAU protein |
Information sourced from Uniprot.org