Human UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1) Sandwich ELISA Kit (STJE0012923)
SPECIFICATIONS
General Information
| Short Description | This UGT1A1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.51ng/mL |
| Detection Limit | 1.56-100ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | UGT1A1 |
| Gene ID | 54658 |
| Uniprot ID | UD11_HUMAN |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Isoform 1: Expressed in liver, colon and small intestine. Not expressed in kidney, esophagus and skin. Isoform 2: Expressed in liver, colon, small intestine and kidney. Not expressed in esophagus and skin. |
| Post Translational Modifications | |
| Function | Isoform 1: UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol, estrone and estriol. Involved in the glucuronidation of bilirubin, a degradation product occurring in the normal catabolic pathway that breaks down heme in vertebrates. Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, 20-HETE, PGB1 and F2-isoprostane (8-iso-PGF2alpha). Involved in the glucuronidation of the phytochemical ferulic acid at the phenolic or the carboxylic acid group. Also catalyzes the glucuronidation the isoflavones genistein, daidzein, glycitein, formononetin, biochanin A and prunetin, which are phytoestrogens with anticancer and cardiovascular properties. Involved in the glucuronidation of the AGTR1 angiotensin receptor antagonist losartan, a drug which can inhibit the effect of angiotensin II. Involved in the biotransformation of 7-ethyl-10-hydroxycamptothecin (SN-38), the pharmacologically active metabolite of the anticancer drug irinotecan. Isoform 2: Lacks UGT glucuronidation activity but acts as a negative regulator of isoform 1. |
| Protein Name | Udp-Glucuronosyltransferase 1a1Ugt1a1Bilirubin-Specific Udpgt Isozyme 1Hug-Br1Udp-Glucuronosyltransferase 1-1Udpgt 1-1Ugt1*1Ugt1-01Ugt1.1Udp-Glucuronosyltransferase 1a Isoform 1 |
| Database Links | Reactome: R-HSA-156588Reactome: R-HSA-189483Reactome: R-HSA-5579002Reactome: R-HSA-9749641Reactome: R-HSA-9753281 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinCytoplasmPerinuclear Region |
| Alternative ELISA Names | Udp-Glucuronosyltransferase 1a1 ELISA kitUgt1a1 ELISA kitBilirubin-Specific Udpgt Isozyme 1 ELISA kitHug-Br1 ELISA kitUdp-Glucuronosyltransferase 1-1 ELISA kitUdpgt 1-1 ELISA kitUgt1*1 ELISA kitUgt1-01 ELISA kitUgt1.1 ELISA kitUdp-Glucuronosyltransferase 1a Isoform 1 ELISA kitUGT1A1 ELISA kitGNT1 ELISA kitUGT1 ELISA kit |
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Information sourced from Uniprot.org