Human UBA5 protein (Recombinant) (N-His) (STJP014813)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human UBA5-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Supplied as a 0.22 Mu m filtered solution in 20mM Tris-HCl, 50mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
Target Information
| Gene Symbol | UBA5 |
| Gene ID | 79876 |
| Uniprot ID | UBA5_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Met404 |
Additional Info
| Function | E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation plays a key role in various processes, such as ribosome recycling, response to DNA damage, interferon response or reticulophagy (also called ER-phagy). Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme 5Ubiquitin-Activating Enzyme 5Thifp1Ufm1-Activating EnzymeUbiquitin-Activating Enzyme E1 Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic Reticulum MembraneGolgi ApparatusLocalizes Mainly In The CytoplasmWhile It Localizes To The Nucleus In Presence Of Sumo2Interaction With Gabarapl2 Promotes Localization To The Endoplasmic Reticulum Membrane |
| Alternative Protein Names | Ubiquitin-Like Modifier-Activating Enzyme 5 proteinUbiquitin-Activating Enzyme 5 proteinThifp1 proteinUfm1-Activating Enzyme proteinUbiquitin-Activating Enzyme E1 Domain-Containing Protein 1 proteinUBA5 proteinUBE1DC1 protein |
Information sourced from Uniprot.org