Human TRIM72 protein (Recombinant) (N-His) (STJP014757)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human TRIM72-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | TRIM72 |
| Gene ID | 493829 |
| Uniprot ID | TRI72_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Arg278-Val470 |
Additional Info
| Function | Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles. |
| Protein Name | Tripartite Motif-Containing Protein 72Mitsugumin-53Mg53 |
| Database Links | Reactome: R-HSA-445355 |
| Cellular Localisation | Cell MembraneSarcolemmaCytoplasmic Vesicle MembraneTethered To Plasma Membrane And Cytoplasmic Vesicles Via Its Interaction With Phosphatidylserine |
| Alternative Protein Names | Tripartite Motif-Containing Protein 72 proteinMitsugumin-53 proteinMg53 proteinTRIM72 proteinMG53 protein |
Information sourced from Uniprot.org