Human Transthyretin/TTR protein (Recombinant) (C-His) (STJP001067)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Transthyretin/TTR Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gly 21-Glu 147 ) of human Transthyretin (Accession #NP_000362.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Transthyretin/TTR-C-His protein was developed in hek293 cells using the region Gly 21-Glu 147. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | TTR |
| Gene ID | 7276 |
| Uniprot ID | TTHY_HUMAN |
| Immunogen | Recombinant Human Transthyretin/TTR Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gly 21-Glu 147 ) of human Transthyretin (Accession #NP_000362.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Gly 21-Glu 147 |
| Immunogen Sequence | GPTGTGESKCPLMVKVLDAV RGSPAINVAVHVFRKAADDT WEPFASGKTSESGELHGLTT EEEFVEGIYKVEIDTKSYWK ALGISPFHEHAEVVFTANDS GPRRYTIAALLSPYSYSTTA VVTNPKE |
Additional Info
| Tissue Specificity | Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver. |
| Post Translational Modifications | Not glycosylated under normal conditions. Following unfolding, caused for example by variant AMYLD1 'Gly-38', the cryptic Asn-118 site is exposed and glycosylated by STT3B-containing OST complex, leading to its degradation by the ER-associated degradation (ERAD) pathway. Sulfonation of the reactive cysteine Cys-30 enhances the stability of the native conformation of TTR, avoiding misassembly of the protein leading to amyloid formation. |
| Function | Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. |
| Protein Name | TransthyretinAttrPrealbuminTbpa |
| Database Links | Reactome: R-HSA-2453902Reactome: R-HSA-3000171Reactome: R-HSA-6798695Reactome: R-HSA-975634Reactome: R-HSA-977225Reactome: R-HSA-9918449 |
| Cellular Localisation | SecretedCytoplasm |
| Alternative Protein Names | Transthyretin proteinAttr proteinPrealbumin proteinTbpa proteinTTR proteinPALB protein |
Information sourced from Uniprot.org