Human Transferrin/TF protein (Recombinant) (C-His) (STJP002681)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Transferrin/TF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val20-Pro698) of human Transferrin (Accession #NP_001054.1) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Transferrin/TF-C-His protein was developed in hek293 cells using the region Val20-Pro698. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 0.075-0.3 ng/mL.|2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant human Transferrin at 2 |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | TF |
| Gene ID | 7018 |
| Uniprot ID | TRFE_HUMAN |
| Immunogen | Recombinant Human Transferrin/TF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val20-Pro698) of human Transferrin (Accession #NP_001054.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Val20-Pro698 |
| Immunogen Sequence | VPDKTVRWCAVSEHEATKCQ SFRDHMKSVIPSDGPSVACV KKASYLDCIRAIAANEADAV TLDAGLVYDAYLAPNNLKPV VAEFYGSKEDPQTFYYAVAV VKKDSGFQMNQLRGKKSCHT GLGRSAGWNIPIGLLYCDLP EPRKPLEKAVANFFSGSCAP CADGTDFPQLCQLCPGCGCS TLNQYFGYSGAFKCLKDGAG DVAFVKHSTIFENLANKADR DQYELLCLDNTRKPVDEYK |
Additional Info
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6:ESAG7 and extract its iron for its own use. |
| Protein Name | SerotransferrinTransferrinBeta-1 Metal-Binding GlobulinSiderophilin |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-381426Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8957275Reactome: R-HSA-917937Reactome: R-HSA-917977 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Serotransferrin proteinTransferrin proteinBeta-1 Metal-Binding Globulin proteinSiderophilin proteinTF proteinPRO1400 protein |
Information sourced from Uniprot.org