Human TNNI3 (Troponin I, cardiac muscle) CLIA Kit for Quantitative Detection

Name: Human TNNI3 (Troponin I, cardiac muscle) CLIA Kit for Quantitative Detection
SKU: STJC0000352
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SPECIFICATIONS

STJC0000352

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Short Description	This human TNNI3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	46.88pg/mL
Detection Limit	78.13~5000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	TNNI3
Gene ID	7137
Uniprot ID	TNNI3_HUMAN
Specificity	This kit recognizes Human cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Human cTn-I/TNNI3 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Function	Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Name	Troponin I - Cardiac Muscle Cardiac Troponin I
Database Links	Reactome: R-HSA-390522 Reactome: R-HSA-5578775
Cellular Localisation
Alternative CLIA Names	Troponin I - Cardiac Muscle CLIA kit Cardiac Troponin I CLIA kit TNNI3 CLIA kit TNNC1 CLIA kit
Specificity	This kit recognizes Human cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Human cTn-I/TNNI3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	254.53	513.41	1903.30	257.46	557.97	1898.33
Standard deviation	24.89	60.02	156.64	30.05	44.30	184.52
CV (%)	9.78	11.69	8.23	11.67	7.94	9.72