| Short Description : | Recombinant-Human TNFRSF10B/DR5/TRAIL-R/2CD262-C-His protein was developed in hek293 cells using the region Ile 56-Glu 182. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | 1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human TRAIL at 2 Mu g/mL (100 Mu L/well) can bind Recombinant Human DR5 with a linear range of 14-56 ng/mL.|2.Measured by its ability to inhibit TRAIL-mediated cytotoxic |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | TNFRSF10B |
| Gene ID: | 8795 |
| Uniprot ID: | TR10B_HUMAN |
| Immunogen: | Active Recombinant Human TNFRSF10B/DR5/TRAIL-R2 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ile 56-Glu 182) of human DR5 (Accession #NP_003833.3) fused with a 6×His tag at the C-terminus. |
| Immunogen Region: | Ile 56-Glu 182 |
| Immunogen Sequence: | ITQQDLAPQQRAAPQQKRSS PSEGLCPPGHHISEDGRDCI SCKYGQDYSTHWNDLLFCLR CTRCDSGEVELSPCTTTRNT VCQCEEGTFREEDSPEMCRK CRTGCPRGMVKVGDCTPWSD IECVHKE |
| Post Translational Modifications | (Microbial infection) Glycosylated on Arg residue by S.typhimurium protein Ssek3. |
| Function | Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 10bDeath Receptor 5Tnf-Related Apoptosis-Inducing Ligand Receptor 2Trail Receptor 2Trail-R2Cd Antigen Cd262 |
| Database Links | Reactome: R-HSA-140534Reactome: R-HSA-202733Reactome: R-HSA-3371378Reactome: R-HSA-5213460Reactome: R-HSA-5218900Reactome: R-HSA-6803211Reactome: R-HSA-69416Reactome: R-HSA-75158 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Tumor Necrosis Factor Receptor Superfamily Member 10b proteinDeath Receptor 5 proteinTnf-Related Apoptosis-Inducing Ligand Receptor 2 proteinTrail Receptor 2 proteinTrail-R2 proteinCd Antigen Cd262 proteinTNFRSF10B proteinDR5 proteinKILLER proteinTRAILR2 proteinTRICK2 proteinZTNFR9 proteinUNQ160 proteinPRO186 protein |
Information sourced from Uniprot.org
