Human TLR-2 (Toll-Like Receptor 2) Antibody Pair (STJAP00181)
General Information
| Short Description | This TLR-2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TLR2 |
| Gene ID | 7097 |
| Uniprot ID | TLR2_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human TLR-2 protein.Detection Immunogen: Recombinant Human TLR-2 protein. |
Additional Info
| Post Translational Modifications | Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. Ubiquitinated at Lys-754 by PPP1R11, leading to its degradation. Deubiquitinated by USP2. |
| Function | Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4. MAPK activation in response to bacterial peptidoglycan also occurs via this receptor. Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36).the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen. M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression. Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides. Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM. |
| Protein Name | Toll-Like Receptor 2Toll/Interleukin-1 Receptor-Like Protein 4Cd Antigen Cd282 |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-1461957Reactome: R-HSA-166058Reactome: R-HSA-168179Reactome: R-HSA-168188Reactome: R-HSA-5602498Reactome: R-HSA-5603041Reactome: R-HSA-5686938Reactome: R-HSA-6798695Reactome: R-HSA-9637628Reactome: R-HSA-9705671Reactome: R-HSA-9833110 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCytoplasmic VesiclePhagosome MembraneMembrane RaftDoes Not Reside In Lipid Rafts Before Stimulation But Accumulates Increasingly In The Raft Upon The Presence Of The Microbial LigandIn Response To Diacylated LipoproteinsTlr2:Tlr6 Heterodimers Are Recruited In Lipid RaftsThis Recruitment Determines The Intracellular Targeting To The Golgi ApparatusTriacylated Lipoproteins Induce The Same Mechanism For Tlr2:Tlr1 Heterodimers |
| Alternative Antibody Pair Names | Toll-Like Receptor 2 antibody pairToll/Interleukin-1 Receptor-Like Protein 4 antibody pairCd Antigen Cd282 antibody pairTLR2 antibody pairTIL4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human TLR-2 (Toll-Like Receptor 2) Antibody Pair (STJAP00181)
This antibody pair detects human TLR-2 (Toll-Like Receptor 2), corresponding to UniProt entry O60603. Reactivity has been confirmed for human samples only.
Both antibodies are polyclonal, raised in goat, and supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant human TLR-2 protein. Both antibodies have also been purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.