Human Tissue factor pathway inhibitor protein (Recombinant) (STJP001876)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding mature chain of human Tissue factor pathway inhibitor-TFPI (ASP29-LYS282) including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Tissue factor pathway inhibitor-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2ยฐC-8ยฐC C for one month, or at-20ยฐC to-70ยฐC for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). NA |
| Immunoreactivity | The activity was tested by the ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRKDnp-NH2, with a calculated ED50 < 3.5nM NA |
Target Information
| Gene Symbol | TFPI |
| Gene ID | 7035 |
| Uniprot ID | TFPI1_HUMAN |
| Immunogen | Optimized DNA sequence encoding mature chain of human Tissue factor pathway inhibitor-TFPI (ASP29-LYS282) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ASP29-LYS282 |
Additional Info
| Tissue Specificity | Mostly in endothelial cells. |
| Post Translational Modifications | O-glycosylated. |
| Function | Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. |
| Protein Name | Tissue Factor Pathway InhibitorTfpiExtrinsic Pathway InhibitorEpiLipoprotein-Associated Coagulation InhibitorLaci |
| Database Links | Reactome: R-HSA-140834 |
| Cellular Localisation | Isoform Alpha: SecretedIsoform Beta: Microsome MembraneLipid-AnchorGpi-Anchor |
| Alternative Protein Names | Tissue Factor Pathway Inhibitor proteinTfpi proteinExtrinsic Pathway Inhibitor proteinEpi proteinLipoprotein-Associated Coagulation Inhibitor proteinLaci proteinTFPI proteinLACI proteinTFPI1 protein |
Information sourced from Uniprot.org