Human TIMP-1 protein (Recombinant) (STJP000306)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding mature chain of human Metalloproteinase inhibitor 1-TIMP-1 (CYS24-ALA207 ) including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human TIMP-1-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by the ability of recombinant human TIMP2 to inhibit human MMP2 cleavage of a fluorogenic peptide substrate, the calculated ED50 is 2nM. NA |
Target Information
| Gene Symbol | TIMP2 |
| Gene ID | 7077 |
| Uniprot ID | TIMP2_HUMAN |
| Immunogen | Optimized DNA sequence encoding mature chain of human Metalloproteinase inhibitor 1-TIMP-1 (CYS24-ALA207 ) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | CYS24-ALA207 |
Additional Info
| Post Translational Modifications | The activity of TIMP2 is dependent on the presence of disulfide bonds. |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. |
| Protein Name | Metalloproteinase Inhibitor 2Csc-21kTissue Inhibitor Of Metalloproteinases 2Timp-2 |
| Database Links | Reactome: R-HSA-1592389Reactome: R-HSA-6798695Reactome: R-HSA-9839383 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Metalloproteinase Inhibitor 2 proteinCsc-21k proteinTissue Inhibitor Of Metalloproteinases 2 proteinTimp-2 proteinTIMP2 protein |
Information sourced from Uniprot.org