Human TIMP-1 protein (Recombinant) (C-His) (STJP001333)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human TIMP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Ala207) of human TIMP-1/CLGI (Accession #NP_003245.1) fused with 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human TIMP-1-C-His protein was developed in hek293 cells using the region Cys24-Ala207. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TIMP1 Protein at 1 Mu g/mL (100 Mu L/well) can bind TIMP1 Rabbit mAb with a linear range of 0.038-2.5 ng/mL.|2.Measured by its ability to inhibit human MMP-2 cleavage of a flu |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | TIMP1 |
| Gene ID | 7076 |
| Uniprot ID | TIMP1_HUMAN |
| Immunogen | Recombinant Human TIMP-1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys24-Ala207) of human TIMP-1/CLGI (Accession #NP_003245.1) fused with 6×His tag at the C-terminus. |
| Immunogen Region | Cys24-Ala207 |
| Immunogen Sequence | CTCVPPHPQTAFCNSDLVIR AKFVGTPEVNQTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVCGYFHRSHNRS EEFLIAGKLQDGLLHITTCS FVAPWNSLSLAQRRGFTKTY TVGCEECTVFPCLSIPCKLQ SGTHCLWTDQLLQGSEKGFQ SRHLACLPREPGLCTWQSLR SQIA |
Additional Info
| Tissue Specificity | Detected in rheumatoid synovial fluid (at protein level). |
| Post Translational Modifications | The activity of TIMP1 is dependent on the presence of disulfide bonds. N-glycosylated. |
| Function | Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro.but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. |
| Protein Name | Metalloproteinase Inhibitor 1Erythroid-Potentiating ActivityEpaFibroblast Collagenase InhibitorCollagenase InhibitorTissue Inhibitor Of Metalloproteinases 1Timp-1 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1592389Reactome: R-HSA-381426Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-8957275Reactome: R-HSA-9839383 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Metalloproteinase Inhibitor 1 proteinErythroid-Potentiating Activity proteinEpa proteinFibroblast Collagenase Inhibitor proteinCollagenase Inhibitor proteinTissue Inhibitor Of Metalloproteinases 1 proteinTimp-1 proteinTIMP1 proteinCLGI proteinTIMP protein |
Information sourced from Uniprot.org