Human TIGIT protein (Recombinant) (C-His-Avi) {Biotin} (STJP001722)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenDNA sequence encoding Human TIGIT including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
General Information
| Short Description | Recombinant-Human TIGIT-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM |
| Formulation | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). |
Target Information
| Gene Symbol | TIGIT |
| Gene ID | 201633 |
| Uniprot ID | TIGIT_HUMAN |
| Immunogen | DNA sequence encoding Human TIGIT including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Immunogen Region | Met22-Pro141 |
Additional Info
| Function | Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Also acts as a receptor for NECTIN4 to inhibit NK cell cytotoxicity. |
| Protein Name | T-Cell Immunoreceptor With Ig And Itim DomainsV-Set And Immunoglobulin Domain-Containing Protein 9V-Set And Transmembrane Domain-Containing Protein 3 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinClustered To The Immunological Synapse Where It Disrupts Granule Polarization And Cytotoxicity Of Nk Cells Once Engaged With Pvr |
| Alternative Protein Names | T-Cell Immunoreceptor With Ig And Itim Domains proteinV-Set And Immunoglobulin Domain-Containing Protein 9 proteinV-Set And Transmembrane Domain-Containing Protein 3 proteinTIGIT proteinVSIG9 proteinVSTM3 protein |
Information sourced from Uniprot.org