Human Thrombopoietin R/MPL/CD110 protein (Recombinant) (C-His) (STJP020353)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Thrombopoietin R/MPL/CD110 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Trp491) of human Thrombopoietin R/MPL (Accession #NP_005364.1) fused with a 6×His tag at the C-te
General Information
| Short Description | Recombinant-Human Thrombopoietin R/MPL/CD110-C-His protein was developed in hek293 cells using the region Gln26-Trp491. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MPL |
| Gene ID | 4352 |
| Uniprot ID | TPOR_HUMAN |
| Immunogen | Recombinant Human Thrombopoietin R/MPL/CD110 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln26-Trp491) of human Thrombopoietin R/MPL (Accession #NP_005364.1) fused with a 6×His tag at the C-te |
| Immunogen Region | Gln26-Trp491 |
| Immunogen Sequence | QDVSLLASDSEPLKCFSRTF EDLTCFWDEEEAAPSGTYQL LYAYPREKPRACPLSSQSMP HFGTRYVCQFPDQEEVRLFF PLHLWVKNVFLNQTRTQRVL FVDSVGLPAPPSIIKAMGGS QPGELQISWEEPAPEISDFL RYELRYGPRDPKNSTGPTVI QLIATETCCPALQRPHSASA LDQSPCAQPTMPWQDGPKQT SPSREASALTAEGGSCLISG LQPGNSYWLQLRSEPDGIS |
Additional Info
| Tissue Specificity | Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed. |
| Post Translational Modifications | Phosphorylated at Tyr-591 in response to THPO stimulation. Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination. |
| Function | Receptor for thrombopoietin that regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. Upon activation by THPO, induces rapid tyrosine phosphorylation and activation of JAK2, providing docking sites for many signaling proteins such as STAT5, SHIP/INPP5D, GRB2, SOS1 and PI3K. In turn, These signaling cascades lead to the proliferation, survival, and differentiation of megakaryocytes, ultimately leading to increased platelet production. |
| Protein Name | Thrombopoietin ReceptorTpo-RMyeloproliferative Leukemia ProteinProto-Oncogene C-MplCd Antigen Cd110 |
| Database Links | Reactome: R-HSA-76009 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinGolgi ApparatusCell Surface |
| Alternative Protein Names | Thrombopoietin Receptor proteinTpo-R proteinMyeloproliferative Leukemia Protein proteinProto-Oncogene C-Mpl proteinCd Antigen Cd110 proteinMPL proteinTPOR protein |
Information sourced from Uniprot.org