Human THP (Uromodulin) Antibody Pair (STJAP00423)
General Information
| Short Description | This THP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Sheep |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | UMOD |
| Gene ID | 7369 |
| Uniprot ID | UROM_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human THP protien.Detection Immunogen: Recombinant Human THP protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. N-glycan heterogeneity at Asn-232: Hex7HexNAc6 (major) and dHex1Hex7HexNAc6 (minor).at Asn-322: dHex1Hex6HexNAc5 (minor), dHex1Hex7HexNAc6 (major) and dHex1Hex8HexNAc7 (minor).at Asn-396: Hex6HexNAc5 (major), dHex1Hex6HexNAc5 (minor) and Hex7HexNAc6 (minor). Glycosylated Asn-232 interacts with E.coli adhesin fimH. Other complex glycosylation sites may serve as binding sites for proteins from other bacteria inclduding K.pneumoniae, P.aeruginosa and S.mitis. Proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in urine. This cleavage is catalyzed by HPN. |
| Function | Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability (Probable). May serve as a receptor for binding and endocytosis of cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia. Uromodulin, secreted form: In the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, and inhibits formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Protects against urinary tract infections by binding to type 1 fimbriated E.coli. Binds to bacterial adhesin fimH which mediates the stable formation of bacterial aggregates, prevents the binding of E.coli to uroplakins UPK1A and UPK1B which act as urothelial receptors for type I fimbriae, and allows for pathogen clearance through micturation. Also promotes aggregation of other bacteria including K.pneumoniae, P.aeruginosa and S.mitis and so may also protect against other uropathogens. |
| Protein Name | UromodulinTamm-Horsfall Urinary GlycoproteinThp Cleaved Into - Uromodulin - Secreted Form |
| Database Links | Reactome: R-HSA-446203 |
| Cellular Localisation | Apical Cell MembraneLipid-AnchorGpi-AnchorBasolateral Cell MembraneCell ProjectionCilium MembraneOnly A Small Fraction Sorts To The Basolateral Pole Of Tubular Epithelial Cells Compared To Apical LocalizationSecreted Into Urine After CleavageColocalizes With Nphp1 And Kif3aUromodulinSecreted Form: SecretedDetected In Urine |
| Alternative Antibody Pair Names | Uromodulin antibody pairTamm-Horsfall Urinary Glycoprotein antibody pairThp Cleaved Into - Uromodulin - Secreted Form antibody pairUMOD antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human THP (Uromodulin) Antibody Pair (STJAP00423)
This antibody pair detects human THP (Uromodulin), corresponding to UniProt entry P07911, and is validated for reactivity against human samples only.
The capture antibody is unconjugated and mouse-hosted, while the detection antibody is biotin-conjugated and sheep-hosted. Both are supplied at 0.5 mg/mL.
This pair is validated for use in ELISA. Suitable sample types include serum, plasma, and other biological fluids.
Both the capture and detection antibodies were raised against recombinant human THP protein. The capture antibody was purified by Protein A or G, while the detection antibody underwent antigen affinity purification.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.