Human Thioredoxin/SASP/TXN protein (Recombinant) (C-His) (STJP000811)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human Thioredoxin/SASP/TXN Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val2-Val105) of human Thioredoxin (Accession #NP_003320.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Thioredoxin/SASP/TXN-C-His protein was developed in e.coli using the region Val2-Val105. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TXN Protein at 1 Mu g/mL (100 Mu L/well) can bind TXN Rabbit mAb with a linear range of 0.976-5.3 ng/mL.|2.Measured by its ability to catalyze the reduction of insulin. The re |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | TXN |
| Gene ID | 7295 |
| Uniprot ID | THIO_HUMAN |
| Immunogen | Recombinant Human Thioredoxin/SASP/TXN Protein is produced by E. coli expression system. The target protein is expressed with sequence (Val2-Val105) of human Thioredoxin (Accession #NP_003320.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Val2-Val105 |
| Immunogen Sequence | VKQIESKTAFQEALDAAGDK LVVVDFSATWCGPCKMIKPF FHSLSEKYSNVIFLEVDVDD CQDVASECEVKCMPTFQFFK KGQKVGEFSGANKEKLEATI NELV |
Additional Info
| Post Translational Modifications | In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. |
| Function | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). |
| Protein Name | ThioredoxinTrxAtl-Derived FactorAdfSurface-Associated Sulphydryl ProteinSaspAllergen Hom S Trx |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-3299685Reactome: R-HSA-499943Reactome: R-HSA-5628897Reactome: R-HSA-5676934Reactome: R-HSA-844456Reactome: R-HSA-9617629Reactome: R-HSA-9660826Reactome: R-HSA-9818027 |
| Cellular Localisation | NucleusCytoplasmSecretedTranslocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma)Predominantly In The Cytoplasm In Non Irradiated CellsRadiation Induces Translocation Of Trx From The Cytoplasm To The NucleusSecreted By A Leaderless Secretory Pathway |
| Alternative Protein Names | Thioredoxin proteinTrx proteinAtl-Derived Factor proteinAdf proteinSurface-Associated Sulphydryl Protein proteinSasp proteinAllergen Hom S Trx proteinTXN proteinTRDX proteinTRX proteinTRX1 protein |
Information sourced from Uniprot.org