Human Thioredoxin Reductase 1/TXNRD1 protein (Recombinant) (His-Tag) (STJP016710)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Thioredoxin Reductase 1/TXNRD1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is > 15unit/mg, and was measured in a coupled assay with 5, 5-Dithiobis (2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm. |
Target Information
| Gene Symbol | TXNRD1 |
| Gene ID | 7296 |
| Uniprot ID | TRXR1_HUMAN |
| Accession Number | NP_001087240.1 |
| Immunogen | Human |
| Immunogen Region | 161-647aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Isoform 1: Expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4: Widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. |
| Post Translational Modifications | Isoform 5: The N-terminus is blocked. ISGylated. |
| Function | Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2). Isoform 1: Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4: Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2. Isoform 5: Enhances the transcriptional activity of the estrogen receptor ESR2 only. Mediates cell death induced by a combination of interferon-beta and retinoic acid. |
| Protein Name | Thioredoxin Reductase 1 - CytoplasmicTrGene Associated With Retinoic And Interferon-Induced Mortality 12 ProteinGrim-12Gene Associated With Retinoic And Ifn-Induced Mortality 12 ProteinKm-102-Derived Reductase-Like FactorPeroxidase Txnrd1Thioredoxin Reductase Tr1 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2408550Reactome: R-HSA-3299685Reactome: R-HSA-499943Reactome: R-HSA-5263617Reactome: R-HSA-5336415Reactome: R-HSA-5628897Reactome: R-HSA-9818027 |
| Cellular Localisation | Isoform 1: CytoplasmIsoform 4: CytoplasmNucleusIsoform 5: Cytoplasm |
| Alternative Protein Names | Thioredoxin Reductase 1 - Cytoplasmic proteinTr proteinGene Associated With Retinoic And Interferon-Induced Mortality 12 Protein proteinGrim-12 proteinGene Associated With Retinoic And Ifn-Induced Mortality 12 Protein proteinKm-102-Derived Reductase-Like Factor proteinPeroxidase Txnrd1 proteinThioredoxin Reductase Tr1 proteinTXNRD1 proteinGRIM12 proteinKDRF protein |
Information sourced from Uniprot.org