Human TG (Thyroglobulin) Antibody Pair (STJAP00114)
General Information
| Short Description | This TG antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TG |
| Gene ID | 7038 |
| Uniprot ID | THYG_HUMAN |
| Immunogen | Capture immunogen: Native Protein.Detection Immunogen: Native Protein. |
Additional Info
| Post Translational Modifications | Iodinated on tyrosine residues by TPO. There are 4 pairs of iodinated tyrosines used for coupling: acceptor Tyr-24 is coupled to donor Tyr-149 or Tyr-234, acceptor Tyr-2573 is coupled to donor Tyr-2540, acceptor Tyr-2766 in monomer 1 is coupled to donor Tyr-2766 in monomer 2 and acceptor Tyr-1310 in monomer 1 is coupled to donor Tyr-108 in monomer 2. Sulfated tyrosines are desulfated during iodination. Undergoes sequential proteolysis by cathepsins to release thyroxine (T4) and triiodothyronine (T3) hormones. In the thyroid follicle lumen, cross-linked TG (storage form) is solubilized by limited proteolysis mediated by cathepsins CTSB and/or CTSL. Partially cleaved TG is further processed by CTSK/cathepsin K and/or CTSL resulting in the release of T4. Following endocytosis, further processing occurs leading to the release of T3 and more T4 hormones. |
| Function | Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules. |
| Protein Name | ThyroglobulinTg |
| Database Links | |
| Cellular Localisation | SecretedSecreted Into The Thyroid Follicle LumenLocalizes To Colloid GlobulesA Structure Formed In The Thyroid Follicle Lumen Consisting Of Cross-Linked Tg Arranged In Concentric Layers |
| Alternative Antibody Pair Names | Thyroglobulin antibody pairTg antibody pairTG antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human TG (Thyroglobulin) Antibody Pair (STJAP00114)
This antibody pair detects human Thyroglobulin (TG), corresponding to UniProt accession P01266. Reactivity is confirmed for human samples only.
Both antibodies are rabbit polyclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotin-conjugated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair has been validated for use in ELISA. No other assay formats are specified for this product.
Both antibodies were purified by antigen affinity purification. The immunogen for each was the native human Thyroglobulin protein.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.