Human TFAM protein (Recombinant) (His-Tag) (STJP017035)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human TFAM-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TFAM |
| Gene ID | 7019 |
| Uniprot ID | TFAM_HUMAN |
| Accession Number | NP_003192.1 |
| Immunogen | Human |
| Immunogen Region | 43-246aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease. |
| Function | Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. |
| Protein Name | Transcription Factor A - MitochondrialMttfaMitochondrial Transcription Factor 1Mttf1Transcription Factor 6Tcf-6Transcription Factor 6-Like 2 |
| Database Links | Reactome: R-HSA-163282Reactome: R-HSA-2151201Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionMitochondrion MatrixMitochondrion Nucleoid |
| Alternative Protein Names | Transcription Factor A - Mitochondrial proteinMttfa proteinMitochondrial Transcription Factor 1 proteinMttf1 proteinTranscription Factor 6 proteinTcf-6 proteinTranscription Factor 6-Like 2 proteinTFAM proteinTCF6 proteinTCF6L2 protein |
Information sourced from Uniprot.org