Human TDP43 (TDP43) Antibody Pair (STJAP00827)
General Information
| Short Description | This TDP43 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R98-7T-3/R95-6N-1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | TARDBP |
| Gene ID | 23435 |
| Uniprot ID | TADBP_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. |
| Post Translational Modifications | Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress. Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. |
| Function | RNA-binding protein that is involved in various steps of RNA biogenesis and processing. Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs. In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases. Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts. Also regulates mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening. In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival. Also participates in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins. Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner. Negatively regulates the expression of CDK6. Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner. |
| Protein Name | Tar Dna-Binding Protein 43Tdp-43 |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmStress GranuleMitochondrionContinuously Travels In And Out Of The NucleusLocalizes To Stress Granules In Response To Oxidative StressA Small Subset Localizes In Mitochondria |
| Alternative Antibody Pair Names | Tar Dna-Binding Protein 43 antibody pairTdp-43 antibody pairTARDBP antibody pairTDP43 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human TDP43 (TDP43) Antibody Pair (STJAP00827)
This antibody pair detects human TDP43 (UniProt Q13148) and is validated for reactivity in human samples only. Reactivity in other species has not been confirmed for this product.
Both antibodies are supplied as unconjugated, affinity-purified rabbit monoclonal IgGs (clone IDs R98-7T-3 and R95-6N-1) at a concentration of 1 mg/mL each, formulated in 0.01M PBS at pH 7.2.
This pair is validated for use in ELISA. No other assay formats are listed as validated applications for this product.
Optimal working dilutions for both antibodies are to be determined by the end user. Titration is recommended when setting up the assay in a specific experimental context.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.