Human TCEB1 protein (Recombinant) (His-Tag) (STJP017486)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human TCEB1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1mM DTT, 0.15M NaCl. |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELOC |
| Gene ID | 6921 |
| Uniprot ID | ELOC_HUMAN |
| Accession Number | NP_005639 |
| Immunogen | Human |
| Immunogen Region | 1-112aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MDGEEKTYGG CEGPDAMYVK LISSDGHEFI VKREHALTSG TIKAMLSGPG QFAENETNEV NFREIPSHVL SKVCMYFTYK VRYTNSSTEI PEFPIAPEIA LELLMAANFL DC |
Additional Info
| Tissue Specificity | Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. |
| Post Translational Modifications | Ubiquitinated by the DCX(AMBRA1) complex, leading to its degradation by the proteasome. |
| Function | SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. Component the von Hippel-Lindau ubiquitination complex CBC(VHL). A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The ECS(ASB9) complex mediates ubiquitination and degradation of CKB. As part of a multisubunit ubiquitin ligase complex, polyubiquitinates monoubiquitinated POLR2A. ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase. As part of the ECS(RAB40C) complex, mediates ANKRD28 ubiquitination and degradation, thereby inhibiting protein phosphatase 6 (PP6) complex activity and focal adhesion assembly during cell migration. (Microbial infection) Following infection by HIV-1 virus, component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, which catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G. The complex can also ubiquitinate APOBEC3H to some extent. |
| Protein Name | Elongin-CElocElongin 15 Kda SubunitRna Polymerase Ii Transcription Factor Siii Subunit CSiii P15Transcription Elongation Factor B Polypeptide 1 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-1234176Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-180585Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-75955Reactome: R-HSA-8951664Reactome: R-HSA-9010553Reactome: R-HSA-9705462Reactome: R-HSA-983168Reactome: R-HSA-9833109 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Elongin-C proteinEloc proteinElongin 15 Kda Subunit proteinRna Polymerase Ii Transcription Factor Siii Subunit C proteinSiii P15 proteinTranscription Elongation Factor B Polypeptide 1 proteinELOC proteinTCEB1 protein |
Information sourced from Uniprot.org