Human TBP2 (Thioredoxin Binding Protein 2) Sandwich ELISA Kit (STJE0003193)

SPECIFICATIONS
STJE0003193
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General Information

Short DescriptionThis TBP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity0.09ng/mL
Detection Limit0.16~10ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolTXNIP
Gene ID10628
Uniprot IDTXNIP_HUMAN
SpecificityThis kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Function May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
Protein Name Thioredoxin-Interacting Protein
Thioredoxin-Binding Protein 2
Vitamin D3 Up-Regulated Protein 1
Database Links Reactome: R-HSA-844456
Reactome: R-HSA-9617629
Reactome: R-HSA-9660826
Reactome: R-HSA-9707564
Cellular Localisation Cytoplasm
Alternative ELISA Names Thioredoxin-Interacting Protein ELISA kit
Thioredoxin-Binding Protein 2 ELISA kit
Vitamin D3 Up-Regulated Protein 1 ELISA kit
TXNIP ELISA kit
VDUP1 ELISA kit
Specificity This kit recognizes Human TBP2 in samples. No significant cross-reactivity or interference between Human TBP2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-106 97
EDTA plasma (n=8) 94-111 101
Cell culture media (n=8) 89-102 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.46 1.15 4.20 0.44 1.13 3.90
Standard deviation 0.03 0.06 0.17 0.03 0.06 0.16
CV (%) 6.52 5.22 4.05 6.82 5.31 4.10

Citations

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