Human SYNPO (Synaptopodin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human SYNPO kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | SYNPO |
| Gene ID | 11346 |
| Uniprot ID | SYNPO_HUMAN |
| Specificity | This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in cerebral cortex. |
| Post Translational Modifications | O-glycosylated. |
| Function | Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity. |
| Protein Name | Synaptopodin |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionTight JunctionPerikaryonCell ProjectionDendritic SpinePostsynaptic DensitySynapseCytosolLocalized At The Tight Junction Of CellsIn BrainLocalized To The Postsynaptic Densities And In The PerikaryaAssociated With Dendritic Spines Of A Subset Of Synapses |
| Alternative CLIA Names | Synaptopodin CLIA kitSYNPO CLIA kitKIAA1029 CLIA kit |
| Specificity | This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-111 | 105 |
| EDTA plasma(n=8) | 96-112 | 102 |
| Cell culture media(n=8) | 89-103 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 108.03 | 170.04 | 869.89 | 106.51 | 169.09 | 869.70 |
| Standard deviation | 11.99 | 19.52 | 65.94 | 13.62 | 19.09 | 66.10 |
| CV (%) | 11.10 | 11.48 | 7.58 | 12.79 | 11.29 | 7.60 |